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PAXgene Blood RNA Kit(配套PAXgene采血/RNA保存管提取血液RNA)

PAX Blood RNA Kit用于配套 PAXgene Blood RNA Tubes使用,PAXgene Blood RNA Tubes采集血样后可以在18–25°C条件下可稳定多至3天,在–20°C或–70°C条件下至少稳定60个月。

产品编号:2112

产品规格:50次

产品价格:2160

包装:

储存条件:4℃

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PAXgene Blood RNA Kit  配套PAXgene采血RNA保存管提取血液RNA(离心柱型) 


BD 762165  PAXgene Blood RNA tube

Blood RNA 试剂盒配套的全血RNA采血管,本公司有多款品牌现货,欢迎配套采购!全血RNA采血管 + PAXgene Blood RNA Kit,批量配套采购,价格更优!欢迎邮件咨询:service@laqqu.com,发邮件请留联系方式!


BD 762165  PAXgene Blood RNA tube 静脉真空采血管(全血RNA管)  
货号: 762165  
规格:100管/盒
含票价格:12000元/盒

散装价格:4500元/30支,3000元/20支

签合同先付款后发货!


产品介绍:
PAX Blood RNA Kit用于配套 PAXgene Blood RNA Tubes使用,PAXgene Blood RNA Tubes采集血样后可以在18–25°C条件下可稳定多至3天,在–20°C或–70°C条件下至少稳定60个月。本产品可以提取保存在采血管的全血中分离和纯化胞间RNA,与采样、稳定及纯化形成整合的系统。PAX Blood RNA系统包括用于血液采集、稳定和运输的PAXgene Blood RNA Tubes(由BD提供,货号762165),以及基于硅胶膜技术、离心柱法分离纯化的PAX Blood RNA Kit。纯化可使用离心机进行手工操作,也可在QIAcube全自动核酸纯化仪上自动运行。该产品表现卓越,确保高度可靠的RNA纯化。

适用范围:

适用于配套 PAXgene Blood RNA Tubes使用,提取保存在采血管的全血中分离和纯化胞间RNA。


Description
PAX Blood RNA Kit is designed for isolation of total RNA from blood samples stored in preservation reagents and PAXgene® Blood RNA Tubes. This procedure completely removes contaminants and enzyme inhibitors producing high-quality RNA. RNA purified using the PAX Blood RNA Kit is ready for applications such as RT-PCR.

The samples are removed from the preservation reagents. For blood samples stored in PAXgene® Blood RNA Tubes, the cells are collected by centrifugation. Samples are washed and lysed under an optimized buffer containing Proteinase K. The samples are centrifuged to remove cell debris and other particulates. After adjusting the binding conditions with ethanol, the samples are loaded on the RNA binding column. With a brief centrifugation or vacuum step, the samples pass through the column matrix which binds the RNA. Genomic DNA is removed with an on-the-column DNase I digestion treatment. After three wash steps, purified RNA is eluted with RNase-free water.

Materials and Equipment to be Supplied by User:
1.Microcentrifuge capable of 13,000 x g
2.100% ethanol
3.RNase-free filter pipette tips
4.RNase-free water
5.1.5 or 2.0 ml microcentrifuge tubes
6.Shaking incubators or heat blocks capable of 55°C, 65°C
7.Centrifuge with swing-bucket rotor capable of 5,500 x g

Procedure
Note:
Before the first use, add the indicated amount of ethanol into Wash Buffer RW bottles, mix well, and mark the bottle with a check.
Heat the incubators or heat blocks to 55°C, 65°C.

1.Centrifuge the PAXgene® Blood RNA Tube for 10 minutes at 3,000-5,000 x g using a swing-out rotor.
2.Aspirate and discard the supernatant.
3.Add 4 mL RNase-free water. Vortex to completely resuspended the pellet.
4.Centrifuge the PAXgene® Blood RNA Tube for 10 minutes at 3,000-5,000 x g using a swing-out rotor.
5.Aspirate and discard the supernatant.
Note: Incomplete removal of the supernatant will reduce the lysis efficiency and dilute the lysate, thereby reducing the RNA yield.
6.Add 350 µl Resuspension buffer. Vortex to completely resuspend the pellet.
7.Transfer the sample into a new 1.5 ml microcentrifuge tube.
8.Add 300 μl Binding Buffer and 20 μL Proteinase K Solution. Vortex for 5 seconds to mix thoroughly.
9.Incubate at 55°C for 10 minutes using a shaking incubator.
10.Optional: Pass the lysate at least 5 times through a 20-gauge needle (0.9 mm diameter) fitted to an syringe or homogenize with an electronic tissue homogenizer. This step shears genomic DNA, reduces the viscosity of the lysates, and increases the yields.
11.Centrifuge the homogenized lysate at 13,000 rpm for 3 min. Transfer the supernatant into a new centrifuge tube. 
12.Add 0.5 volumes 100% ethanol. Vortex to mix thoroughly.
13.Transfer up to 700 µl mixture into a RNA binding column placed in a 2 ml collection tube (provided). 
14.Centrifuge at maximum speed for 1 minute.
15.Aspirate and discard the filtrate and reuse the collection tube.
16.Repeat Steps 13-15 until the remaining sample has been transferred to RNA binding column.
17.Add 350 μl Buffer RW1. 
18.Centrifuge at maximum speed for 1 minute. 
19.Aspirate and discard the filtrate and reuse the collection tube.
20.For each of the RNA binding column, prepare the DNase I digestion reaction mix as follows:

Buffer 
Volume per Prep
10 Preps
DNase I Buffer
 45 μl 
450 μl
RNase-free DNase I
 5 μl
 50 μl
Total volume
 50 μl 
500 μl
Important Notes:
• DNase I is very sensitive and prone to physical denaturing. Do not vortex the DNase I mixture. Mix gently by inverting the tube.
• Freshly prepare DNase I stock solution right before RNA isolation.
• Standard DNase buffers are not compatible with on-membrane DNase I digestion. The use of other buffers may affect the binding of RNA to the matrix and may reduce RNA yields and purity.
• All steps must be carried out at room temperature. Work quickly, but carefully.
21.Pipet 50 μl DNase I digestion reaction mix directly onto the centre surface of the RNA binding column.
Note: make sure to pipet the DNase I digestion mixture directly onto the membrane. DNase I digestion will not be complete if some of the mixture is retained on the wall or o-ring of the RNA binding column 
22.Let sit at room temperature for 15 minutes.
23.Add 350 μl Buffer RW1. Centrifuge at maximum speed for 1 minute.
24.Aspirate and discard the filtrate and reuse the collection tube.
25.Add 500 µl Wash Buffer RW. Centrifuge at maximum speed for 1 minute.
26.Aspirate and discard the filtrate and reuse the collection tube.
27.Repeat steps 25-26 for a second Wash Buffer RW wash step.
28.Centrifuge the empty RNA binding column for 2 minutes at maximum speed to dry the column matrix.
Note: It is important to dry the column membrane before elution. Residual ethanol may interfere with downstream applications.
29.Transfer the RNA binding column into a 1.5 mL microcentrifuge tube.
30.Add 50-70 μl RNase-free water(Optional: pre-warm the water to 70–90C will increase the RNA yield) directly onto the center of the membrane. Let sit at room temperature for 1 minute.
31.Centrifuge at maximum speed for 2 minutes.


我们生产的试剂盒同Q762174,PAXgene Blood RNA Kit Handbook,操作步骤一样,提取得率一样,提取效果一样,价格更实惠全国包邮!使用不满意包退!

Qiagene762174和Genenode2112实验结果比较

Qiagene762174和Genenode2112实验结果比较


具体产品折扣价请邮件或电话咨询!


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我们还承接各种组织样品RNA提取,反转录,实时荧光定量PCR检测服务! 链接:点击



mRNA检测原价120元,推广促销价75元/基因/3个重复;

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根据具体样品数量,组织RNA提取难度,酌情收取少量RNA提取费用,内参免费;


技术优势:

1. 各种组织样品RNA提取试剂盒的研发生产和提取经验;

2. 专业的引物设计技能,保证qPCR引物的特异性;

3. 熟练的qPCR操作技能,保证完美的qPCR结果;

qPCR实验流程图

送样要求:

1. 细胞(≥106 )、新鲜动植物组织(≥300mg)、血液(≥1ml)、叶片(≥100mg)等样品材料,基因组DNA或总RNA(总RNA>2μg,体积≥20μl,浓度≥100 ng/μl)。
2. 靶基因信息和背景资料:基因序列或GenBank Accession Number等,生物物种信息(Human、Mouse、Rat 等)、DNA/RNA 来源、基因丰度等。

提供服务:
引物探针设计合成,RNA提取,反转录,RNA电泳,引物筛选,上机定量检测。

提交结果:
原始数据(CT值和各种统计值,融化温度图,扩增曲线图,电泳图,柱状图)、数据分析结果及详细实验报告。